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Microsynth’s special treatment service is especially useful if you want to sequence constructs with problematic structures. These constructs often have GC-rich regions and/or build hairpin structures (e.g. DNA coding for siRNA).
Generally, we recommend the following approaches to our customers:
Over the years Microsynth has developed robust methods for the sequencing of large DNA constructs such as BACs, cosmids and fosmids. Please choose the DNA type accordingly in our webshop.
Microsynth’s one-drop sequencing service allows you to sequence a plasmid when you don’t have sufficient amounts of DNA. By applying this type of service, we are able to perform sequencing if you provide us with as little as 50 ng in at least 1 µl of your sample. This procedure takes one additional day.
You send us your E. coli samples as single colonies on agar plates. We pick the desired number of clones and take care of isolation and sequencing. Plasmids, isolated on demand, will be sent back to you, if requested in higher scales (midi/maxi).