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RNA Oligonucleotides

 
 
Microsynth synthesis facilities enable fast and reliable assembly of RNA oligonucleotides. Chimeric DNA/RNA oligomers, including siRNA sequences, are rapidly available from our fully-integrated synthesis robots.   
Furthermore you can select from a wide range of modifications to label or functionalize your RNA sequence.
 

Features and Benefits

 
 
High Quality
  • Stringent quality control (online trityl monitoring and  MALDI-TOF MS or analytical PAGE)
  • Renowned supplier for top research institutes and pharma/biotech companies
 
Fast Production
  • Unmodified oligos: ≤ 2 d
  • Modified oligos: ≤ 3 d
 
Excellent Technical Support
  • Trained scientists with molecular biology background are happy to support you (from 8 am to 5 pm)
 
 
 
Cost Effective
  • Very competitive pricing for volume as well as repetitive orders
  • High guaranteed yields and/or average delivery yields
 
Comprehensive Service Portfolio
  • Large Range of different synthesis scales up to large-scale
  • Broad spectrum of high quality modifications and purification
 
User-friendly Online Ordering System
  • New and easy-to-use online portal with a series of helpful tools (e.g. order tracking & history, convenient search and re-order option)

Synthesis Scales

 

Microsynth is offering its RNA oligos at 5 different scales. For large scale synthesis please get in contact with our experts.
The available standard scales are Genomics, 0.04 µmol, 0.2 µmol, 1.0 µmol and 15 µmol.
As the yield and length restrictions depend also on the purification used please have a look at the tables below.

 

Desalted RNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol 10 - 30 4 21 6 28 2
0.2 µmol 10 - 504 8 35 10 45 2
1.0 µmol 10 - 504 18 80 22 100 2
15 µmol 10 - 40 400 1'800 800 2'200 3
 
HPLC Purified RNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol 10 - 30 1 5 2 10 2
0.2 µmol 10 - 80 3 15 5 25 2
1.0 µmol 13 65 17 85 2
15 µmol 10 - 40 300 1'500 360 1'800 4
 
HPLC Purified & Dialysed RNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol not available
0.2 µmol 10 - 80 2 10 3 15 4
1.0 µmol 9 45 11 55 4
15 µmol 10 - 40 200 1'000 250 1'250 4
 
PAGE Purified RNA Oligos
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol not available
0.2 µmol 10 - 504 1 5 1 5 2
1.0 µmol 10 - 504 6 30 7 30 2
15 µmol not available

 

1 The synthesis scale represents the initial amount of 3' bases (starting material).

2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20 and <40 nucleotides.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 RNA bases.

4 Oligos longer than 50 RNA nucleotides can be produced in combination with HPLC purification.

Modifications

 

Microsynth offers a wide variety of modifications (5’, 3’ and internal) for DNA oligonucleotides. To give you a better overview, they have been categorized as: 

To learn more about the key specifications of a modification, please look at Modifications.

 

How to Order

 

  • Enter our webshop
  • Click on Single Stranded RNA in the "Oligonucleotide" domain
  • Select Normal Entry in order to type or copy/paste the desired sequence information etc. and follow the further instructions